The fourth Global Microbiome Network online symposium encompassed talks from the fields of microbiology, molecular biology, ecology, bioinformatics, anthropology, conservation, preservation, ethics, and public health. The symposium discussed and promoted the value of microbial biodiversity and the importance of conservation efforts. GloMiNe for Chile was hosted by the Pontificia Universidad Católica de Chile. This is an initiative under the mission of Microbiota Vault to conserve long-term health for humanity and the environment where we live.